Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1)
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產(chǎn)品名稱: Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1)
產(chǎn)品型號(hào): T0134
產(chǎn)品展商: ABM
產(chǎn)品文檔: 無相關(guān)文檔
簡單介紹
Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) 上海 安徽 合肥 ABM細(xì)胞庫
Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1)
的詳細(xì)介紹
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Print Version
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BioSafety Level
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II
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Organism
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Leptin receptor-deficient mouse (db/db)
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Source Organ
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Bone Marrow
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Donor Gender
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Male
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Growth Properties
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Adherent
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Morphology
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Polygonal
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Recommended Seeding Density
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Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
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Applications
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For Research Use Only
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Immortalization Method
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Spontaneous immortalization
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Description
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The Immortalized Mouse Leptin Receptor-deficient Macrophage Cells (DB-1) are non-transformed cells derived from leptin-receptor (LepR) deficient mice which display some phenotypic and functional properties similar to primary macrophages. The cells are characterized as early differentiated macrophages since the high expression of MAC-2, and low expression of F4/80, Ly6G and Ly6C were observed; but CD11b expression was absent. It is recommended for use in studies involving the role of the leptin receptor, susceptibility to infections, innate immune functions, and obesity.
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Procedure Overview
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Image
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Image
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Propagation
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Use of PriCoat? T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow III medium available from ABM (TM003). To make the completed growth medium, add the following components to the base medium: a final concentration of 10% fetal bovine serum (TM999), 10% Opti-MEM (gibco), 15 mM/L HEPES, and 100 μg/ml gentamycin or 1% Penicillin/Streptomycin (G255). Atmosphere: air: 95%, CO?: 5%; Temperature: 37.0°C.
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Quality Control
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1) qRT-PCR confirmed LepR deficiency; 2) Flow cytometry was used to analyze cell surface markers.
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Disclaimer
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1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
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公司簡介
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合肥星肽生物科技有限公司作為專業(yè)的ABM中國代理商,專業(yè)經(jīng)營原裝進(jìn)口胎牛血清、細(xì)胞因子、ELISA試劑盒、細(xì)胞、抗體、生物試劑、耗材、培養(yǎng)基、一抗、二抗等。ABM安徽代理的產(chǎn)品涉及分子生物學(xué)、細(xì)胞生物學(xué)、細(xì)菌學(xué)、遺傳學(xué)、免疫學(xué)、生物化學(xué)、蛋白質(zhì)學(xué)、細(xì)胞治療、臨床應(yīng)用等領(lǐng)域。合肥星肽生物科技有限公司始終致力于為客戶提供可靠的研究材料以及方便快捷的服務(wù),對(duì)購買項(xiàng)目的前期資料提供,中期合同保證,后期貨物跟蹤到*終售后的確保項(xiàng)目準(zhǔn)確到位,都有相關(guān)專業(yè)人士進(jìn)行維護(hù),確保您在合肥合肥星肽生物科技有限公司公司獲得*上等服務(wù)!
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聯(lián)系方式
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何經(jīng)理,合肥星肽生物科技有限公司,Tel:0551-63802898 400-8702-898,
E-mail:info@qingbio.com http://m.qycsh.com QQ:514713116
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